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    Mixed-mode Stationary Phases

    For decades, liquid chromatography stationary phase design has been dominated by the idea of elimination of multiple, or “unwanted” interactions that occur in mixed-mode separations. Reverse-phase chromatography is making the point of eliminating silanol interactions with amine-containing analytes by developing base-deactivated phases.

    Within the same trend, adsorption interactions are considered undesirable as opposed to steric interactions in size-exclusion chromatography, and non-ionic interactions are generally viewed as complications in ion-exchange and ion-exclusion chromatography.

    However, there are ways to benefit from multiple interactions on the stationary phase. SIELC Technologies introduces Primesep® HPLC columns, specifically designed for mixed-mode separations and capable of separating a tremendous range of compounds by different separation modes based only upon mobile phase selection. MORE

    Currently, SIELC Technologies offers the following types of silica-based mixed-mode phases.

     Primesep A - Acidic
    Primesep A - Acidic
     Primesep B - Basic
    Primesep B - Basic

    Primesep C - Complex
    Primesep C - Complex

    Primesep P - Phenyl
    Primesep P - Phenyl
    Columns with negatively charged functional groups due to the embedded anionic ion-pairing reagent.
    Primesep A
    Strongest acidic phase
    Primesep 100
    Strong acidic phase
    Primesep 200
    Medium acidic phase

    Choose the correct column by their functional group acidity to match the pKa value of your basic compounds. MORE

    Columns with positively charged functional groups. Primesep B
    Strong basic column for use with TFA, phosphoric or perchloric acid solutions in a mobile phase. Primesep B2
    Weak basic column for ammonium acetate buffered solution. MORE
    Column with unique unparalleled selectivity. Primesep C
    Combined reverse-phase and complex formation properties for interaction with amines, sulfonium, phosphonium and metal ions. MORE
     Three interactions within one column:
    • Reverse phase
    • Pi-pi
    • Strong cation exchange

    Primesep P
    Choose for separation of structural isomers of aromatic compounds.

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